| 4. | Nature. 2014 May 29;509(7502):575-81. doi: 10.1038/nature13302.Kim MS1, Pinto SM2, Getnet D3, Nirujogi RS2, Manda SS2, Chaerkady R1, Madugundu AK2, Kelkar DS2, Isserlin R4, Jain S4, Thomas JK2, Muthusamy B2, Leal-Rojas P5, Kumar P2, Sahasrabuddhe NA2, Balakrishnan L2, Advani J2, George B2, Renuse S2, Selvan LD2, Patil AH2, Nanjappa V2, Radhakrishnan A2, Prasad S6, Subbannayya T2, Raju R2, Kumar M2, Sreenivasamurthy SK2, Marimuthu A2, Sathe GJ2, Chavan S2, Datta KK2, Subbannayya Y2, Sahu A2, Yelamanchi SD2, Jayaram S2, Rajagopalan P2, Sharma J2, Murthy KR2, Syed N2, Goel R2, Khan AA2, Ahmad S2, Dey G2, Mudgal K7, Chatterjee A2, Huang TC6, Zhong J6, Wu X1, Shaw PG6, Freed D6, Zahari MS8, Mukherjee KK9, Shankar S10, Mahadevan A11, Lam H12, Mitchell CJ6, Shankar SK11, Satishchandra P13, Schroeder JT14, Sirdeshmukh R2, Maitra A15, Leach SD16, Drake CG17, Halushka MK18, Prasad TS2, Hruban RH15, Kerr CL19, Bader GD4, Iacobuzio-Donahue CA20, Gowda H2, Pandey A21. Author information:
11] McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA [2] Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
2Institute of Bioinformatics, International Tech Park, Bangalore 560066, India.
31] McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA [2] Adrienne Helis Malvin Medical Research Foundation, New Orleans, Louisiana 70130, USA.
4The Donnelly Centre, University of Toronto, Toronto, Ontario M5S 3E1, Canada.
51] McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA [2] Department of Pathology, Universidad de La Frontera, Center of Genetic and Immunological Studies-Scientific and Technological Bioresource Nucleus, Temuco 4811230, Chile.
6McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
7School of Medicine, Imperial College London, South Kensington Campus, London SW7 2AZ, UK.
8Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
9Department of Neurosurgery, Postgraduate Institute of Medical Education & Research, Chandigarh 160012, India.
10Department of Internal Medicine Armed Forces Medical College, Pune 411040, India.
111] Department of Neuropathology, National Institute of Mental Health and Neurosciences, Bangalore 560029, India [2] Human Brain Tissue Repository, Neurobiology Research Centre, National Institute of Mental Health and Neurosciences, Bangalore 560029, India.
12Department of Chemical and Biomolecular Engineering and Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong.
13Department of Neurology, National Institute of Mental Health and Neurosciences, Bangalore 560029, India.
14Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21224, USA.
151] The Sol Goldman Pancreatic Cancer Research Center, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA [2] Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA.
161] McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA [2] Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA.
171] Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA [2] Departments of Immunology and Urology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA.
18The Sol Goldman Pancreatic Cancer Research Center, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA.
191] Department of Obstetrics and Gynecology, Johns Hopkins University School of Medicine Baltimore, Maryland 21205, USA [2] Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA.
201] The Sol Goldman Pancreatic Cancer Research Center, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA [2] Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA [3] Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA.
211] McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA [2] Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA [3] Institute of Bioinformatics, International Tech Park, Bangalore 560066, India [4] Adrienne Helis Malvin Medical Research Foundation, New Orleans, Louisiana 70130, USA [5] The Sol Goldman Pancreatic Cancer Research Center, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA [6] Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA [7] Diana Helis Henry Medical Research Foundation, New Orleans, Louisiana 70130, USA. AbstractThe availability of human genome sequence has transformed biomedical research over the past decade. However, an equivalent map for the human proteome with direct measurements of proteins and peptides does not exist yet. Here we present a draft map of the human proteome using high-resolution Fourier-transform mass spectrometry. In-depth proteomic profiling of 30 histologically normal human samples, including 17 adult tissues, 7 fetal tissues and 6 purified primary haematopoietic cells, resulted in identification of proteins encoded by 17,294 genes accounting for approximately 84% of the total annotated protein-coding genes in humans. A unique and comprehensive strategy for proteogenomic analysis enabled us to discover a number of novel protein-coding regions, which includes translated pseudogenes, non-coding RNAs and upstream open reading frames. This large human proteome catalogue (available as an interactive web-based resource at http://www.humanproteomemap.org) will complement available human genome and transcriptome data to accelerate biomedical research in health and disease. |
